Stage: 5 Challenge Level:
The best way of approaching the restriction map is to first draw a
circle. Next draw on the cutting points for one of the restriction
enzymes. Consider the second restriction enzyme and see
if you can place the cutting sites such that they are the correct
size for both enzymes together, but also satisfy the second enzyme
on its own. There may be several possibilities at these points. Use
the data for the third restriction enzyme to eliminate all the
possibilities bar one.
Note that some restriction maps are not unique, in that the data
given may allow more than one valid map to be drawn.