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A standard calibration for a spectrophotometric assay for lysozyme was created by diluting some initial concentrations of the reagents Tris HCl buffer, NaCl, Lysozyme and a Micrococcus lysodeikticus suspension.The final volume was 5ml.
The numbers in the calculation have become mixed up. Can you work out how consistently to fill in the table? There will be two numbers left over. What will these represent?
Here is a printable version of the table and cards that you can cut out.
|
|
Initial conc. |
Final conc. |
Dilution factor |
Volume added |
|
Tris |
|
|
|
|
|
NaCl |
|
|
|
|
|
Lysozyme |
|
|
|
|
|
Cells |
|
|
|
|
|
171 mM
|
106 /ml |
10mM |
500 pmol/ml |
|
0.050 ml
|
136 |
20 |
0.250 ml |
|
68 nmol/ml
|
50 mM |
100 |
0.037 ml |
|
0.292 ml
|
108 /ml |
17.1 |
0.629 ml |
|
4.371 ml
|
1000 mM |
|
|