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Light Absorbance

Stage: 5 Challenge Level: Challenge Level:3 Challenge Level:3 Challenge Level:3

This question contains a lot of 'unused' information, so try to be clear as to which parts enter the calculation.

You might want to think through the problem in these steps:

First consider how the molar extinction coefficient relates to the absorbance of the initial solution in the light path cuvette using the Beer-Lambert law.

Then work out how a change in this absorbance relates to a change in the concentration. From this you can work out the change in the number of moles of chemical due to the sample of extract added to the cuvette.

Then you can scale up to determine the number of katals present in the original extract.