You may also like

problem icon

Epidemic Modelling

Use the computer to model an epidemic. Try out public health policies to control the spread of the epidemic, to minimise the number of sick days and deaths.

problem icon

bioNRICH

bioNRICH is the area of the stemNRICH site devoted to the mathematics underlying the study of the biological sciences, designed to help develop the mathematics required to get the most from your study of biology at A-level and university.

problem icon

Conversion Sorter

Can you break down this conversion process into logical steps?

Mixed up Mixture

Stage: 4 Challenge Level: Challenge Level:1



The solution is shown above, but is little help without the logical thought process which allowed its deduction.

Firstly, looking at the mass of data, it is notable that of the concentrations, two of them are given solely as a number per ml, rather than in moles per ml or l. These clearly match up with the 'cells' row, as it is common to talk about the number of cells per ml, rather than as a molar concentration. It is immediately clear that the $10^8$ must be the initial concentration and the $10^6$ value the final concentration, since a dilution occurs. This dilution factor is 100, as given by $\frac{10^8}{10^6}$. It is now possible to match these three pieces of data with the necessary 'volume added'. Dividing the total 5ml volume by the dilution factor gives an added volume of 0.050ml.

Matching the rest of the data is somewhat trickier, and it is perhaps useful to organise the data into three piles: concentrations, numbers, and volumes. Dilution factors can be coupled to pairs of concentrations through observation or trial and error. This reveals the two unused volumes, which can be put to one side.

It should hopefully be evident that Lysozyme is an enzyme, which is a protein formed from many amino acids. It thus has an exceptionally large RMM compared to either NaCl or Tris, and so its molar concentration is likely to be much smaller than either of these. Therefore, Lysozyme can be matched with the 68 nmol/ml, 500pmol/ml 136 and 0.037ml category.

Matching the final data to the Tris and NaCl is much trickier. One way of deducing this is to look up the cellular concentration of sodium ions, since the NaCl may be being added to the assay to ensure that the solution is isotonic, and so the cells will not lose or gain water by osmosis. Research reveals that these sodium ion concentrations are between 5 and 15 mMol, and so it is likely that data set with the final concentration of 10 mMol matches with NaCl.

Therefore, the final data set must match with Tris-Hcl!

The final part of the questions asks what the two unused volumes correspond to. 0.629ml corresponds to the total volume of the 4 solutions added so far to the assay, and 4.371ml corresponds to the amount of water that should be added to make up the solution to 5ml.